Simple fluorometric-based assay of antibiotic effectiveness for Acinetobacter baumannii biofilms.

ABSTRACT: Despite strengthened antimicrobial therapy, biofilm infections of Acinetobacter baumannii are associated with poor prognosis and limited therapeutic options. Assessing antibiotics on planktonic bacteria can result in failure against biofilm infections. Currently, antibiotics to treat biofilm infections are administered empirically, usually without considering the susceptibility of the biofilm objectively before beginning treatment. For effective therapy to resolve biofilm infections it is essential to assess the efficacy of commonly used antibiotics against biofilms. Here, we offer a robust and simple assay to assess the efficacy of antibiotics against biofilms. In the present work, we carefully optimized the incubation time, detection range, and fluorescence reading mode for resazurin-bas

SUBMITTER: Wannigama DL 

PROVIDER: S-EPMC6474882 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature