Diversity and phylogeny of resveratrol-producing culturable endophytic fungi from Vitis species in India.

ABSTRACT: In the present study, resveratrol producing culturable endophytes were recovered from the Indian vineyards. Of the 145 endophytic fungi recovered, only 30.3% were positive for resveratrol production in the preliminary screening. Culture broth of only 13 isolates exhibited the actual presence of resveratrol in the range of 4.4-52.3 ?g/ml by HPLC quantification. The most potent endophytic isolate producing the highest extracellular resveratrol was #19VVLPM isolated from the Pinot Noir variety. Further LC-MS analysis confirmed the similarity of fungal resveratrol with standard resveratrol. The Merlot variety exhibited the highest colonization frequency of the resveratrol-producing fungal endophytes. Using morphological methods as well as molecular phylogeny #19VVLPM was identified as Fusarium equiseti. It is a promising candidate for further development into a commercial strain.

SUBMITTER: Dwibedi V 

PROVIDER: S-EPMC6475630 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature