Heterologous transporters from anaerobic fungi bolster fluoride tolerance in Saccharomyces cerevisiae.

ABSTRACT: Membrane-embedded transporters are crucial for the stability and performance of microbial production strains. Apart from engineering known transporters derived from model systems, it is equally important to identify transporters from nonconventional organisms that confer advantageous traits for biotechnological applications. Here, we transferred genes encoding fluoride exporter (FEX) proteins from three strains of early-branching anaerobic fungi (Neocallimastigomycota) to Saccharomyces cerevisiae. The heterologous transporters are localized to the plasma membrane and complement a fluoride-sensitive yeast strain that is lacking endogenous fluoride transporters up to 10.24 mM fluoride. Furthermore, we show that fusing an amino-terminal leader sequence to FEX proteins in yeast e

SUBMITTER: Seppala S 

PROVIDER: S-EPMC6475669 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature