Metastable Reprogramming State of Single Transcription Factor-Derived Induced Hepatocyte-Like Cells.

ABSTRACT: We previously described the generation of induced hepatocyte-like cells (iHeps) using the hepatic transcription factor Hnf1a together with small molecules. These iHeps represent a hepatic state that is more mature compared with iHeps generated with multiple hepatic factors. However, the underlying mechanism of hepatic conversion involving transgene dependence of the established iHeps is largely unknown. Here, we describe the generation of transgene-independent iHeps by inducing the ectopic expression of Hnf1a using both an episomal vector and a doxycycline-inducible lentivirus. In contrast to iHeps with sustained expression of Hnf1a, transgene-independent Hnf1a iHeps lose their typical morphology and in vitro functionality with rapid downregulation of hep

SUBMITTER: Hwang SI 

PROVIDER: S-EPMC6476006 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature