Expanding and testing fluorescent amplified fragment length polymorphisms for identifying roots of boreal forest plant species.

ABSTRACT:

Premise of the study

Identifying roots to species is challenging, but is a common problem in ecology. Fluorescent amplified fragment length polymorphisms (FAFLPs) can distinguish species within a mixed sample, are high throughput, and are inexpensive. To broaden the use of this tool across ecosystems, unique size profiles must be established for species, and its limits identified.

Methods

Fragments of three noncoding cpDNA regions were used to create size profiles for 193 species common to the western Canadian boreal forest. We compared detection success among congeners using FAFLPs and Sanger sequencing of the trnL intron. We also simulated and experimentally created communities to test the influence of species richness, cpDNA regions used, and extraction/amplificati

SUBMITTER: Metzler P 

PROVIDER: S-EPMC6476169 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature