Marker-assisted transfer of PinaD1a gene to develop soft grain wheat cultivars.

ABSTRACT: Grain softness has been a major trait of interest in wheat because of its role in producing flour suitable for making high-quality biscuits, cookies, cakes and some other products. In the present study, marker-assisted backcross breeding scheme was deployed to develop advanced wheat lines with soft grains. The Australian soft-grained variety Barham was used as the donor parent to transfer the puroindoline grain softness gene Pina-D1a to the Indian variety, DBW14, which is hard grained and has PinaD1bPinbD1a genes. Foreground selection with allele-specific PCR-based primer for Pina-D1a (positive selection) was used to identify heterozygous BC₁F₁ plants. Background selection with 173 polymorphic SSR primers covering all the 21 chromoso

SUBMITTER: Rai A 

PROVIDER: S-EPMC6476891 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature