Leukemic B Cell CTLA-4 Suppresses Costimulation of T Cells.

ABSTRACT: The clinical benefit of CTLA-4 blockade on T cells is known, yet the impact of its expression on cancer cells remains unaddressed. We define an immunosuppressive role for tumor-expressed CTLA-4 using chronic lymphocytic leukemia (CLL) as a disease model. CLL cells, among other cancer cells, are CTLA-4⁺ Coculture with activated human T cells induced surface CTLA-4 on primary human CLL B cells. CTLA-4 on CLL-derived human cell lines decreased CD80 expression on cocultured CD80⁺ cells, with restoration upon CTLA-4 blockade. Coculture of CTLA-4⁺ CLL cells with CD80-GFP⁺ cell lines revealed transfer of CD80-GFP into CLL tumor cells, similar to CTLA-4⁺ T cells able to trans-endocytose CD80. Coculture of T cells with CTLA-4⁺

SUBMITTER: Do P 

PROVIDER: S-EPMC6478536 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature