Aeration and Shear Stress Are Critical Process Parameters for the Production of Oncolytic Measles Virus.

ABSTRACT: Oncolytic Measles virus is a promising candidate for cancer treatment, but clinical studies have shown that extremely high doses (up to 10¹¹ TCID₅₀ per dose) are required to effect a cure. Very high titers of the virus must therefore be achieved during production to ensure an adequate supply. We have previously shown that Measles virus can be produced in Vero cells growing on a Cytodex 1 microcarrier in serum-containing medium using a stirred-tank reactor (STR). However, process optimization and further process transfer or scale up requires the identification of critical process parameters, particularly because the use of STRs increases the risk of cell damage and lower product yields due to shear stress. Using a small-scale STR (0.5 L working volume) we found that Me

SUBMITTER: Grein TA 

PROVIDER: S-EPMC6478815 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature