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Enhanced early-life nutrition upregulates cholesterol biosynthetic gene expression and Sertoli cell maturation in testes of pre-pubertal Holstein bulls.

ABSTRACT: Well-fed prepuberal Holstein bulls had larger testes, earlier puberty, higher LH, testosterone and IGF-1, earlier and more proliferating and differentiating Sertoli cells, and greater sperm production potential. The objective was to determine effects of pre-pubertal nutrition on mRNA expression of testicular genes. Holstein bull calves were fed high or low diets (20 or 12% crude protein, respectively and 71.6 or 64.4% Total Digestible Nutrients) from 2 wk, castrated at 8, 16, 24 and 32 wk and testicular mRNA extracted and sequenced. Differential expression of genes mainly occurred at 16 and 24 wk. At 16 wk, functional analysis (DAVID) of DE mRNA revealed common biological processes including "cholesterol" and "fatty acid biosynthesis," with most genes (including HMGCR, HMGCS1, HSD17) upregulated in high-diet bulls (P?

SUBMITTER: Johnson C

PROVIDER: S-EPMC6478835 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Enhanced early-life nutrition upregulates cholesterol biosynthetic gene expression and Sertoli cell maturation in testes of pre-pubertal Holstein bulls.

Johnson Chinju C Dance Alysha A Kovalchuk Igor I Kastelic John J Thundathil Jacob J

Scientific reports 20190423 1

Well-fed prepuberal Holstein bulls had larger testes, earlier puberty, higher LH, testosterone and IGF-1, earlier and more proliferating and differentiating Sertoli cells, and greater sperm production potential. The objective was to determine effects of pre-pubertal nutrition on mRNA expression of testicular genes. Holstein bull calves were fed high or low diets (20 or 12% crude protein, respectively and 71.6 or 64.4% Total Digestible Nutrients) from 2 wk, castrated at 8, 16, 24 and 32 wk and te ...[more]

PMID: 31015481

Similar Datasets

Enhanced early-life nutrition upregulates cholesterol biosynthetic gene expression and Sertoli cell maturation in testes of pre-pubertal Holstein bulls

Project description:Pre-pubertal Holstein bull calves fed a higher plane of nutrition had larger testes, earlier puberty, higher serum LH, testosterone and greater sperm production potential than those fed a restricted diet. In addition, pre-pubertal calves fed a high-nutrition diet had higher IGF-I and more proliferating and differentiating Sertoli cells much earlier in life, compared to those fed normal or low-nutrition diets. The objective was to determine changes in mRNA expression of genes in the testes of bulls fed either a high or low pre-pubertal diet. Holstein bull calves maintained on either a high (20% crude protein (CP) and 71.6% Total Digestible Nutrients (TDN)) or low (12% CP and 64.4% TDN) diet from 2 wk of age, were castrated at 8, 16, 24 and 32 wk and testicular mRNA extracted and sequenced. Differential expression of genes mainly occurred at 16 and 24 wk, with minor changes detected at 32 wk. At 16 wks, functional analysis of DE mRNA with DAVID revealed the common biological processes enriched to be "cholesterol" and "fatty acid biosynthesis" with majority of the genes including HMGCR, HMGCS1, HSD17 being upregulated in high-diet bulls (P<0.05). Major pathways enriched at 16 wks were "cholesterol biosynthesis", "steroid metabolism" and "activation of gene expression by Sterol regulatory element-binding protein (SREBP)" (P<0.05). Mature Sertoli cell marker Connexin 43, was upregulated at 16 wk, whereas an immature Sertoli cell marker, AMH was downregulated at 24 wk, in the high-diet group. Network analysis using IPA, revealed an indirect interaction between insulin family receptor and most upregulated cholesterol biosynthesis genes, implying regulation of testicular function. Thus, enhanced pre-pubertal nutrition in Holstein bulls enhanced testicular cholesterol/steroid biosynthesis and Sertoli cell maturation to promote early reproductive development.

2019-05-15 | GSE125082 | GEO

Enhanced pre-pubertal nutrition upregulates mitochondrial function in testes and sperm of post-pubertal Holstein bulls.

Project description: Not available

| S-EPMC7010748 | biostudies-literature

Enhanced pre-pubertal nutrition upregulates mitochondrial function in the testes and sperm of post-pubertal Holstein bulls

Project description:Recent studies have documented the profound impact of pre-pubertal nutrition on reproductive performance in bulls. Previously, a high plane of nutrition during calfhood (2-30 wk) in beef and dairy bulls hastened puberty explained by an increase in the level of reproductive hormones (LH, testosterone and IGF-I) pre-pubertally, compared to bulls fed less than recommended amounts of energy and protein with no apparent effect on sperm function evaluated by traditional techniques. In addition, upregulated steroid biosynthesis and Sertoli cell maturation was identified in the testicular tissue of high diet bulls at 16 and 24 weeks, respectively. The objective of this study was to evaluate the post- pubertal testes for responses to high (20.0% CP, 67.9% TDN), medium (17.0% CP, 66.0% TDN) and low (12.2% CP, 62.9% TDN) diets fed from 2-30 wk of life. Based on RNA sequencing data, 497 genes were differentially expressed in high vs low diet and 2961 genes in high vs medium diet (P<0.1). According to KEGG analysis, oxidative phosphorylation and ribosome pathways were upregulated in the high diet group (vs medium and low) with majority of the upregulated genes encoding for essential subunits of complex I, III, IV and V of OXYPHOS pathway. In addition, mitochondrial translation, mitotic nuclear division and cell division were enriched in the high vs medium diet group. Supporting the above, the percentage of sperm exhibiting loss of mitochondrial function was lower in the high diet compared to the medium diet (P<0.1). Thus, enhanced early life nutrition upregulated mitochondrial function in both the testes and sperm of post-pubertal Holstein bulls.

2020-01-01 | GSE137690 | GEO

Enhanced early-life nutrition upregulates cholesterol biosynthetic gene expression and Sertoli cell maturation in testes of pre-pubertal Holstein bulls

Project description:Enhanced early-life nutrition upregulates cholesterol biosynthetic gene expression and Sertoli cell maturation in testes of pre-pubertal Holstein bulls

| PRJNA515100 | ENA

Enhanced pre-pubertal nutrition upregulates mitochondrial function in the testes and sperm of post-pubertal Holstein bulls

Project description:Enhanced pre-pubertal nutrition upregulates mitochondrial function in the testes and sperm of post-pubertal Holstein bulls

| PRJNA566324 | ENA

Hormone induced differential transcriptome analysis of Sertoli cells during postnatal maturation of rat testes.

Project description: Not available

| S-EPMC5771609 | biostudies-literature

Effect of early calf-hood nutrition on the transcriptomic profile of subcutaneous adipose tissue in Holstein-Friesian bulls.

Project description: Not available

| S-EPMC5916831 | biostudies-literature

Effect of early calf-hood nutrition on the transcriptomic profile of subcutaneous adipose in Holstein-Friesian bulls

Project description:The aim of this study was to investigate the effect of early calf-hood nutrition on the transcriptomic profile of subcutaneous adipose in Holstein-Friesian bulls and possible downstream effects on reproduction. Holstein Friesian bull calves with a mean (±S.D.) age and bodyweight of 19 (±8.2) days and 47.5 (±5.3) kg, respectively, were assigned to either a high (n=10) or low (n=10) plane of nutrition. Calves were fed in order to achieve an overall growth rate of 1.08 and 0.5 kg/day for the high and low plane of nutrition treatments, respectively. At 126 days of age, the bulls were euthanized using an intravenous overdose of sodium pentobarbitone and subcutaneous adipose tissue samples were harvested and RNAseq analysis was performed. At the end of the trial period, there were 745 genes differentially expressed in adipose tissue of bulls on the high plane of nutrition relative to those on the low plane of nutrition (P<0.05; FDR<0.05; fold change >2.0). Plane of nutrition altered the expression of genes involved in energy production and branched chain amino acid degradation. Plane of nutrition was also found to down regulate genes such as leptin and adiponectin which are known to effect reproductive function. This study identifies some of the molecular mechanisms regulated by feeding bulls a high plane of nutrition, which may be incorporated into future breeding programmes for artificial insemination centres.

2018-04-12 | GSE97674 | GEO

Core Microbiome of Slovak Holstein Friesian Breeding Bulls' Semen.

Project description: Not available

| S-EPMC8614657 | biostudies-literature

Effect of early calf-hood nutrition on the transcriptomic profile of the HPT axis in Holstein-Friesian bulls

Project description:The aim of this study was to investigate the effect of early calf-hood nutrition on the transcriptomic profile of the arcuate nucleus of the hypothalamus, the anterior pituitary and the testes in Holstein-Friesian bulls and possible downstream effects on reproduction. Holstein Friesian bull calves with a mean (±S.D.) age and bodyweight of 19 (±8.2) days and 47.5 (±5.3) kg, respectively, were assigned to either a high (n=10) or low (n=10) plane of nutrition. Calves were fed in order to achieve an overall growth rate of 1.2 and 0.5 kg/day for the high and low plane of nutrition treatments, respectively. At 126 days of age, the bulls were euthanized using an intravenous overdose of sodium pentobarbitone and arcuate nucleus of the hypothalamus, anterior pituitary and the parenchyma of the testes tissue samples were harvested and RNAseq analysis was performed. At the end of the trial period,

2018-04-12 | GSE97673 | GEO

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