User-defined morphogen patterning for directing human cell fate stratification.

ABSTRACT: Concentration gradients of biochemical stimuli such as morphogens play a critical role in directing cell fate patterning across species and throughout development but are not commonly recapitulated in vitro. While in vitro biomolecule gradients have been generated using customized microfluidic platforms, broad implementation has been limited because these platforms introduce new variables to cell culture such as externally driven flow, culture in a specialized matrix, or extended time for in situ long range diffusion. Here we introduce a method that enables preforming and then transferring user-controlled gradients to cells in standard "open" cultures. Our gradient patterning devices are modular and decoupled from the culture substrate. We find that gradient generation and transfer are pre

SUBMITTER: Regier MC 

PROVIDER: S-EPMC6478938 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature