The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery.

ABSTRACT: Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodology to extract microbial DNA, since biases introduced during the DNA extraction process may result in inaccurate microbial representation. In this study, we attempted to find a DNA extraction protocol which could be effectively used to analyze both the bacterial and fungal community. We evaluated the effect of five DNA extraction methods (QIAamp DNA Stool Mini Kit, PureLink^TM Microbiome DNA Purification Kit, ZR Fecal DNA MiniPrep^TM Kit, NucleoSpin^® DNA Stool Kit, and IHMS protocol Q) on bacterial and fungal gut microbiome recovery using (i) a defined system of ge

SUBMITTER: Fiedorova K 

PROVIDER: S-EPMC6479168 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature