Design of a chromogenic substrate for elastase based on split GFP system-Proof of concept for colour switch sensors.

ABSTRACT: Recent studies have demonstrated that human neutrophil elastase (HNE) can be used as marker for inflammation/infection of chronic wounds since it was found to be present in high concentration in exudate collected from chronic wounds. Biosensors used in wound care benefit from a chromogenic signalling due to the readiness of signal interpretation, but the most common use faint yellow chromogenic molecules such as p-nitroaniline (pNa). In addition, if to be converted into smart dressings, the colour of the detection system should not be masked by the exudate's colour. In this work, we designed a chromogenic substrate for HNE aiming to be incorporated in a smart dressing as a colour switch sensor. The substrate was developed using the GFP-like chromoprotein ultramarine (UM), fol

SUBMITTER: Ferreira AV 

PROVIDER: S-EPMC6479270 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature