Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa.

ABSTRACT: Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed pig spermatozoa retrieved from the cauda epididymis and the ejaculate of the same boars, with clear differences in cryotolerance. Spermatozoa were collected from 10 healthy, sexually mature, and fertile boars, and cryopreserved using a standard 0.5 mL-straw protocol. Total and progressive motility, viability, and mitochondria membrane potential were higher and membrane fluidity and reactive oxygen species generation lower in frozen-thawed (FT) epididymal than ejaculated spermat

SUBMITTER: Perez-Patino C 

PROVIDER: S-EPMC6479301 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature