Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy.

ABSTRACT: Much has been learned about the interaction between myosin and actin through biochemistry, in vitro motility assays and cryo-electron microscopy (cryoEM) of F-actin, decorated with myosin heads. Comparatively less is known about actin-myosin interactions within the filament lattice of muscle, where myosin heads function as independent force generators and thus most measurements report an average signal from multiple biochemical and mechanical states. All of the 3D imaging by electron microscopy (EM) that has revealed the interplay of the regular array of actin subunits and myosin heads within the filament lattice has been accomplished using the flight muscle of the large water bug Lethocerus sp. The Lethocerus flight muscle possesses a particularly favorable filament arrangement that enabl

SUBMITTER: Taylor KA 

PROVIDER: S-EPMC6479483 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature