Electrochemical Enzyme Biosensor Bearing Biochar Nanoparticle as Signal Enhancer for Bisphenol A Detection in Water.

ABSTRACT: An electrochemical tyrosinase enzyme (Tyr) biosensor using a highly conductive sugarcane derived biochar nanoparticle (BCNP) as a transducer and signal enhancer (BCNPs/Tyr/Nafion/GCE) was developed for the sensitive detection of bisphenol A (BPA). The BCNPs/Tyr/Nafion/GCE biosensor exhibited improved amperometric current responses such as higher sensing signal, decreased impedance and lowered reduction potential compared with the Tyr/Nafion/GCE due to high conductivity property of the biochar nanoparticle. Under the optimized conditions, it could detect BPA in good sensitivity with linear range from 0.02 to 10 μM, and a lowest detection limit of 3.18 nM. Moreover, it showed a low Km value, high reproducibility and good selectivity over other reagents, and the BCNPs/Tyr complex solution als

SUBMITTER: Liu Y 

PROVIDER: S-EPMC6479578 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature