Investigating Stability and Tautomerization of Gossypol-A Spectroscopy Study.

ABSTRACT: The stability of gossypol was investigated by the spectroscopic method. Gossypol was dissolved in three different solvents (CHCl₃, DMSO, and CH₃OH) under different storage conditions (dark and with nitrogen protection, natural light and with nitrogen protection, ambient air conditions) for different time intervals (0 days, 3 days, 5 days, 7 days, 15 days, 30 days, and 45 days) at room temperature. Then, the stability of gossypol was investigated by ¹H NMR, UV-vis, and HPLC-QTOF-MS spectrometry. Results showed that gossypol existed in aldehyde-aldehyde form in chloroform within five days. Then, both aldehyde-aldehyde and lactol-lactol tautomeric forms existed and maintained a stable solution for 45 days. Gossypol dissolved in methanol mainly existed in aldehyde-aldehyde form. Only a tiny am

SUBMITTER: Wang L 

PROVIDER: S-EPMC6479638 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature