Metagenomic Profiling of Microbial Pathogens in the Little Bighorn River, Montana.

ABSTRACT: The Little Bighorn River is the primary source of water for water treatment plants serving the local Crow Agency population, and has special significance in the spiritual and ceremonial life of the Crow tribe. Unfortunately, the watershed suffers from impaired water quality, with high counts of fecal coliform bacteria routinely measured during run-off events. A metagenomic analysis was carried out to identify potential pathogens in the river water. The Oxford Nanopore MinION platform was used to sequence DNA in near real time to identify both uncultured and a coliform-enriched culture of microbes collected from a popular summer swimming area of the Little Bighorn River. Sequences were analyzed using CosmosID bioinformatics and, in agreement with previous studies, enterohemorrhagic and ente

SUBMITTER: Hamner S 

PROVIDER: S-EPMC6479903 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature