Brown fat organogenesis and maintenance requires AKT1 and AKT2.

ABSTRACT:

Objective

Understanding the signaling mechanisms that control brown adipose tissue (BAT) development is relevant to understanding energy homeostasis and obesity. The AKT kinases are insulin effectors with critical in vivo functions in adipocytes; however, their role in adipocyte development remains poorly understood. The goal of this study was to investigate AKT function in BAT development.

Methods

We conditionally deleted Akt1 and Akt2 either individually or together with Myf5-Cre, which targets early mesenchymal precursors that give rise to brown adipocytes. Because Myf5-Cre also targets skeletal muscle and some white adipocyte lineages, comparisons were made between AKT function in BAT versus white adipose tissue (WAT) and muscle development. We also deleted both Akt1 and

SUBMITTER: Sanchez-Gurmaches J 

PROVIDER: S-EPMC6480051 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature