Hydrogen-deuterium exchange mass spectrometry of membrane proteins in lipid nanodiscs.

ABSTRACT: Hydrogen deuterium exchange mass spectrometry (H/DX MS) provides a quantitative comparison of the relative rates of exchange of amide protons for solvent deuterons. In turn, the rate of amide exchange depends on a complex combination of the stability of local secondary structure, solvent accessibility, and dynamics. H/DX MS has, therefore, been widely used to probe structure and function of soluble proteins, but its application to membrane proteins was limited previously to detergent solubilized samples. The large excess of lipids from model membranes, or from membrane fractions derived from in vivo samples, presents challenges with mass spectrometry. The lipid nanodisc platform, consisting of apolipoprotein A-derived membrane scaffold proteins, provides a native like membrane environment

SUBMITTER: Redhair M 

PROVIDER: S-EPMC6480397 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature