Cytotoxicity of Human Hepatic Intrasinusoidal CD56^bright Natural Killer Cells against Hepatocellular Carcinoma Cells.

ABSTRACT: Hepatic intrasinusoidal (HI) natural killer (NK) cells from liver perfusate have unique features that are similar to those of liver-resident NK cells. Previously, we have reported that HI CD56^bright NK cells effectively degranulate against SNU398 hepatocellular carcinoma (HCC) cells. Thus, the aim of this study was to further investigate the phenotype and function of HI NK cells. We found that HI CD56^bright NK cells degranulated much less to Huh7 cells. HI CD56^bright NK cells expressed NKG2D, NKp46, TNF-related apoptosis-inducing ligand (TRAIL), and FAS ligand (FASL) at higher levels than CD56^dim cells. SNU398 cells expressed more NKG2D ligands and FAS and less PD-L1 than Huh7 cells. Blockade of NKG2D, TRAIL, and FASL significantly reduced the cy

SUBMITTER: Hwang S 

PROVIDER: S-EPMC6480584 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature