Intratumoral heterogeneity of EGFR-activating mutations in advanced NSCLC patients at the single-cell level.

ABSTRACT:

Background

Intratumoral epidermal growth factor receptor (EGFR) mutational heterogeneity is yet controversial in non-small cell lung cancer (NSCLC) patients. Single-cell analysis provides the genetic profile of single cancer cells and an in-depth understanding of the heterogeneity of a tumor.

Methods

Firstly, single H1975 cells harboring the EGFR L858R mutation were submitted to flow cytometry isolation, nested polymerase chain reaction (nested-PCR) amplification, and direct DNA sequencing to assess the feasibility of single-cell direct DNA sequencing. Then, the single cells of patients with lung adenocarcinoma receiving gefitinib were captured by laser capture microdissection and analyzed by the above methods to identify the intratumoral heterogeneity of the EGFR L858R muta

SUBMITTER: Guo L 

PROVIDER: S-EPMC6480785 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature