Comprehensive characterization of T-DNA integration induced chromosomal rearrangement in a birch T-DNA mutant.

ABSTRACT:

Background

Integration of T-DNA into plant genomes via Agrobacterium may interrupt gene structure and generate numerous mutants. The T-DNA caused mutants are valuable materials for understanding T-DNA integration model in plant research. T-DNA integration in plants is complex and still largely unknown. In this work, we reported that multiple T-DNA fragments caused chromosomal translocation and deletion in a birch (Betula platyphylla × B. pendula) T-DNA mutant yl.

Results

We performed PacBio genome resequencing for yl and the result revealed that two ends of a T-DNA can be integrated into plant genome independently because the two ends can be linked to different chromosomes and cause chromosomal translocation. We also found that these T-DNA were connected into tandem fragment

SUBMITTER: Gang H 

PROVIDER: S-EPMC6480916 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature