A Brief History of Single-Particle Tracking of the Epidermal Growth Factor Receptor.

ABSTRACT: Single-particle tracking (SPT) has been used and developed over the last 25 years as a method to investigate molecular dynamics, structure, interactions, and function in the cellular context. SPT is able to show how fast and how far individual molecules move, identify different dynamic populations, measure the duration and strength of intermolecular interactions, and map out structures on the nanoscale in cells. In combination with other techniques such as macromolecular crystallography and molecular dynamics simulation, it allows us to build models of complex structures, and develop and test hypotheses of how these complexes perform their biological roles in health as well as in disease states. Here, we use the example of the epidermal growth factor receptor (EGFR), which has been studied

SUBMITTER: Clarke DT 

PROVIDER: S-EPMC6481046 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature