Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels.

ABSTRACT: Genetic engineering is considered to be an important tool for the improvement of cassava. Cassava is a highly heterozygous crop species for which conventional breeding is a lengthy and tedious process. Robust transformation is based on Agrobacterium-mediated transformation of friable embryogenic callus (FEC). Production of FEC is genotype-dependent and considered to be a major bottleneck for the genetic transformation of cassava. As a consequence, routine genetic transformation has only been established for a handful of cassava cultivars. Therefore, development of procedures enabling efficient production of high-quality cassava FEC is required to allow the translation of research from the model cultivar to farmer-preferred cassava cultivars. Here we study the FEC production capacity of Bra

SUBMITTER: Lentz EM 

PROVIDER: S-EPMC6481083 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature