Improved Detection and Fragmentation of Disulphide-Linked Peptides.
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ABSTRACT:
SUBMITTER: Maes E
PROVIDER: S-EPMC6481087 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Publications
Improved Detection and Fragmentation of Disulphide-Linked Peptides.
Maes Evelyne E Clerens Stefan S Dyer Jolon M JM Deb-Choudhury Santanu S
Methods and protocols 20180903 3
Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required. ...[more]
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2019-02-01 | PXD011861 | Pride