Improved Detection and Fragmentation of Disulphide-Linked Peptides.

ABSTRACT: Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.

SUBMITTER: Maes E 

PROVIDER: S-EPMC6481087 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature