A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action.

ABSTRACT: Opioid receptors (ORs) precisely modulate behavior when activated by native peptide ligands but distort behaviors to produce pathology when activated by non-peptide drugs. A fundamental question is how drugs differ from peptides in their actions on target neurons. Here, we show that drugs differ in the subcellular location at which they activate ORs. We develop a genetically encoded biosensor that directly detects ligand-induced activation of ORs and uncover a real-time map of the spatiotemporal organization of OR activation in living neurons. Peptide agonists produce a characteristic activation pattern initiated in the plasma membrane and propagating to endosomes after receptor internalization. Drugs produce a different activation pattern by additionally driving OR activation in the somat

SUBMITTER: Stoeber M 

PROVIDER: S-EPMC6481295 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature