The transcriptomic response to irinotecan in colon carcinoma bearing mice preconditioned by fasting.

ABSTRACT:

Background

Irinotecan use is limited due to severe toxicity. Preconditioning by fasting (PBF) protects against side effects of irinotecan while preserving its antitumor activity. The mechanisms underlying the effects of PBF still need to be elucidated. Here, we investigated the transcriptional responses of PBF on irinotecan in both tumor and healthy liver tissue.

Experimental approach

Male BALB/c mice were subcutaneously injected with C26 colon carcinoma cells. Twelve days after tumor inoculation, two groups were fasted for three days and two groups were allowed food ad libitum (AL). Subsequently, both groups received one dose of irinotecan. Twelve hours after administration mice were sacrificed and blood, tumor and liver tissue were harvested. Blood samples were analyzed to

SUBMITTER: Jongbloed F 

PROVIDER: S-EPMC6481335 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature