Hypoxia impacts human MSC response to substrate stiffness during chondrogenic differentiation.

ABSTRACT: Tissue engineering strategies often aim to direct tissue formation by mimicking conditions progenitor cells experience within native tissues. For example, to create cartilage in vitro, researchers often aim to replicate the biochemical and mechanical milieu cells experience during cartilage formation in the developing limb bud. This includes stimulating progenitors with TGF-β_1/3, culturing under hypoxic conditions, and regulating mechanosensory pathways using biomaterials that control substrate stiffness and/or cell shape. However, as progenitors differentiate down the chondrogenic lineage, the pathways that regulate their responses to mechanotransduction, hypoxia and TGF-β may not act independently, but rather also impact one another, influencing overall cell response. Here, to

SUBMITTER: Foyt DA 

PROVIDER: S-EPMC6481516 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature