Mutational landscape of canine B-cell lymphoma profiled at single nucleotide resolution by RNA-seq.

ABSTRACT: The genomic landscape in human B-cell lymphoma has revealed several somatic mutations and potentially relevant germline alterations affecting therapy and prognosis. Also, mutations originally described as somatic aberrations have been shown to confer cancer predisposition when occurring in the germline. The relevance of mutations in canine B-cell lymphoma is scarcely known and gene expression profiling has shown similar molecular signatures among different B-cell histotypes, suggesting other biological mechanisms underlining differences. Here, we present a highly accurate approach to identify single nucleotide variants (SNVs) in RNA-seq data obtained from 62 completely staged canine B-cell lymphomas and 11 normal B-cells used as controls. A customized variant discovery pipeline was applied

SUBMITTER: Giannuzzi D 

PROVIDER: S-EPMC6481796 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature