Sustained Ca2+ mobilizations: A quantitative approach to predict their importance in cell-cell communication and wound healing.

ABSTRACT: Epithelial wound healing requires the coordination of cells to migrate as a unit over the basement membrane after injury. To understand the process of this coordinated movement, it is critical to study the dynamics of cell-cell communication. We developed a method to characterize the injury-induced sustained Ca2+ mobilizations that travel between cells for periods of time up to several hours. These events of communication are concentrated along the wound edge and are reduced in cells further away from the wound. Our goal was to delineate the role and contribution of these sustained mobilizations and using MATLAB analyses, we determined the probability of cell-cell communication events in both in vitro models and ex vivo organ culture models. We demonstrated that the injury response was com

SUBMITTER: Lee Y 

PROVIDER: S-EPMC6481807 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature