A CCRK and a MAK Kinase Modulate Cilia Branching and Length via Regulation of Axonemal Microtubule Dynamics in Caenorhabditis elegans.

ABSTRACT: The diverse morphologies of primary cilia are tightly regulated as a function of cell type and cellular state. CCRK- and MAK-related kinases have been implicated in ciliary length control in multiple species, although the underlying mechanisms are not fully understood. Here, we show that in C. elegans, DYF-18/CCRK and DYF-5/MAK act in a cascade to generate the highly arborized cilia morphologies of the AWA olfactory neurons. Loss of kinase function results in dramatically elongated AWA cilia that lack branches. Intraflagellar transport (IFT) motor protein localization, but not velocities, in AWA cilia is altered upon loss of dyf-18. We instead find that axonemal microtubules are decorated by the EBP-2 end-binding protein along their lengths and that the tubulin load is increased and tubuli

SUBMITTER: Maurya AK 

PROVIDER: S-EPMC6482063 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature