Development and evaluation of a one-step multiplex real-time TaqMan^® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples.

ABSTRACT:

Background

Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and has a negative impact on equine breeding enterprises worldwide. Among ERVA strains infecting foals, the genotypes G3P[12] and G14P[12] are the most prevalent, while infections by strains with other genomic arrangements are infrequent. The identification of circulating strains of ERVA is critical for diagnostic and surveillance purposes, as well as to understand their molecular epidemiology. Current genotyping methods available for ERVA and rotaviruses affecting other animal species rely on Sanger sequencing and are significantly time-consuming, costly and labor intensive. Here, we developed the first one-step multiplex TaqMan^® real-time reverse transcription polymerase chain react

SUBMITTER: Carossino M 

PROVIDER: S-EPMC6482509 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature