Transcriptional profiling of innate immune responses in sheep PBMCs induced by Haemonchus contortus soluble extracts.

ABSTRACT:

Background

Pattern recognition receptors (PRRs) can recognize pathogen-associated molecular patterns and activate downstream signalling pathways, resulting in modulation of host immunity against pathogens. Here, we investigated whether PRR-mediated recognition is involved in host immune responses to the blood-feeding nematode Haemonchus contortus.

Methods

During blood-feeding, H. contortus secretes immune-modulating antigens into host blood. Therefore, we stimulated sheep peripheral blood mononuclear cells (PBMCs) with H. contortus soluble extract (HcAg) and performed transcriptional profiling.

Results

HcAg upregulated two genetically linked CLRs (CLEC2L and KLRG2), two NLRs attenuating inflammation (NLRP12 and NLRC3) and one G protein-coupled receptor with potent ant

SUBMITTER: Wang S 

PROVIDER: S-EPMC6482558 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature