Salinomycin triggers endoplasmic reticulum stress through ATP2A3 upregulation in PC-3 cells.

ABSTRACT:

Background

Salinomycin is a monocarboxylic polyether antibiotic and is a potential chemotherapy drug. Our previous studies showed that salinomycin inhibited cell growth and targeted CSCs in prostate cancer. However, the precise target of salinomycin action is unclear.

Methods

In this work, we analyzed and identified differentially expressed genes (DEGs) after treatment with or without salinomycin using a gene expression microarray in vitro (PC-3 cells) and in vivo (NOD/SCID mice xenograft model generated from implanted PC-3 cells). Western blotting and immunohistochemical staining were used to analyze the expression of ATP2A3 and endoplasmic reticulum (ER) stress biomarkers. Flow cytometry was used to analyze the cell cycle, apoptosis and intracellular Ca²⁺ concen

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6482559 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature