Superoxide dismutase transcellular shuttle constructed from dendritic MOF and charge reversible protein derivatives.

ABSTRACT: The development of molecular biology has led to the identification of protein-based therapeutics as an intriguing approach for the treatment of a wide range of diseases. To manufacture transcellular protein delivery shuttles, we attempted charge reversal chemistry on native proteins [e.g., superoxide dismutase (SOD): an enzyme capable of scavenging detrimental reactive oxygen species] by the selective conversion of the positively charged amino residues of native SOD to conjugated negatively charged citraconic moieties, eliciting overall negatively charged polyelectrolytes for the subsequent electrostatic self-assembly with cationic metal-organic framework (MOF) derivatives into protein delivery systems. Please note that the charge conversion was reversible, restoring the original am

SUBMITTER: Wang W 

PROVIDER: S-EPMC6482591 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature