Quantitative Imaging of Tumor-Associated Macrophages and Their Response to Therapy Using ⁶⁴Cu-Labeled Macrin.

ABSTRACT: Tumor-associated macrophages (TAMs) are widely implicated in cancer progression, and TAM levels can influence drug responses, particularly to immunotherapy and nanomedicines. However, it has been difficult to quantify total TAM numbers and their dynamic spatiotemporal distribution in a non-invasive and translationally relevant manner. Here, we address this need by developing a pharmacokinetically optimized, ⁶⁴Cu-labeled polyglucose nanoparticle (Macrin) for quantitative positron emission tomography (PET) imaging of macrophages in tumors. By combining PET with high-resolution in vivo confocal microscopy and ex vivo imaging of optically cleared tissue, we found that Macrin was taken up by macrophages with >90% selectivity. Uptake correlated with the content of macrophages in both

SUBMITTER: Kim HY 

PROVIDER: S-EPMC6482841 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature