Hepatic differentiation of human pluripotent stem cells by developmental stage-related metabolomics products.

ABSTRACT: Endogenous cell signals regulate tissue homeostasis and are significant for directing the fate of stem cells. During liver development, cytokines released from various cell types are critical for stem/progenitor cell differentiation and lineage expansions. To determine mechanisms in these stage-specific lineage interactions, we modeled potential effects of soluble signals derived from immortalized human fetal liver parenchymal cells on stem cells, including embryonic and induced pluripotent stem cells. For identifying lineage conversion and maturation, we utilized conventional assays of cell morphology, gene expression analysis and lineage markers. Molecular pathway analysis used functional genomics approaches. Metabolic properties were analyzed to determine the extent of hepatic different

SUBMITTER: Bandi S 

PROVIDER: S-EPMC6483088 | biostudies-literature | 2019 Jan - Feb

REPOSITORIES: biostudies-literature