Plant growth-promoting activity of beta-propeller protein YxaL secreted from Bacillus velezensis strain GH1-13.

ABSTRACT: YxaL is conserved within the Bacillus subtilis species complex associated with plants and soil. The mature YxaL protein contains a repeated beta-propeller domain, but the subcellular location and function of YxaL has not been determined. The gene encoding the mature YxaL protein was PCR amplified from genomic DNA of B. velezensis strain GH1-13 and used for recombinant protein production. A rabbit polyclonal antibody against the purified YxaL was generated and used for western blotting to determine the constitutive expression and secretion of YxaL. During normal culture growth of strain GH1-13, levels of the constitutively secreted YxaL were slowly rising to 100 μg L-1, and degraded with a half-life of 1.6 h in the culture medium. When the effects of YxaL on plant seed germination and seedl

SUBMITTER: Kim YH 

PROVIDER: S-EPMC6483160 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature