ISeq 2.0: A Modular and Interchangeable Toolkit for Interaction Screening in Yeast.

ABSTRACT: We developed a flexible toolkit for combinatorial screening in Saccharomyces cerevisiae, which generates large libraries of cells, each uniquely barcoded to mark a combination of DNA elements. This interaction sequencing platform (iSeq 2.0) includes genomic landing pads that assemble combinations through sequential integration of plasmids or yeast mating, 15 barcoded plasmid libraries containing split selectable markers (URA3_AI, KanMX_AI, HphMX_AI, and NatMX_AI), and an array of ∼24,000 "double-barcoder" strains that can make existing yeast libraries iSeq compatible. Various DNA elements are compatible with iSeq: DNA introduced on integrating plasmids, engineered genomic modifications, or entire genetic backgrounds. DNA element libraries are modular

SUBMITTER: Liu X 

PROVIDER: S-EPMC6483859 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature