TRNA Methylation Is a Global Determinant of Bacterial Multi-drug Resistance.

ABSTRACT: Gram-negative bacteria are intrinsically resistant to drugs because of their double-membrane envelope structure that acts as a permeability barrier and as an anchor for efflux pumps. Antibiotics are blocked and expelled from cells and cannot reach high-enough intracellular concentrations to exert a therapeutic effect. Efforts to target one membrane protein at a time have been ineffective. Here, we show that m¹G37-tRNA methylation determines the synthesis of a multitude of membrane proteins via its control of translation at proline codons near the start of open reading frames. Decreases in m¹G37 levels in Escherichia coli and Salmonella impair membrane structure and sensitize these bacteria to multiple classes of antibiotics, rendering them incapable of developing resi

SUBMITTER: Masuda I 

PROVIDER: S-EPMC6483872 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature