Impact of Percoll purification on isolation of primary human hepatocytes.

ABSTRACT: Research and therapeutic applications create a high demand for primary human hepatocytes. The limiting factor for their utilization is the availability of metabolically active hepatocytes in large quantities. Centrifugation through Percoll, which is commonly performed during hepatocyte isolation, has so far not been systematically evaluated in the scientific literature. 27 hepatocyte isolations were performed using a two-step perfusion technique on tissue obtained from partial liver resections. Cells were seeded with or without having undergone the centrifugation step through 25% Percoll. Cell yield, function, purity, viability and rate of bacterial contamination were assessed over a period of 6 days. Viable yield without Percoll purification was 42.4 × 10⁶ (SEM ± 4.6 × 10⁶

SUBMITTER: Horner R 

PROVIDER: S-EPMC6484008 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature