Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex.

ABSTRACT: The development of neural circuits relies on axon projections establishing diverse, yet well-defined, connections between areas of the nervous system. Each projection is formed by growth cones-subcellular specializations at the tips of growing axons, encompassing sets of molecules that control projection-specific growth, guidance, and target selection¹. To investigate the set of molecules within native growth cones that form specific connections, here we developed growth cone sorting and subcellular RNA-proteome mapping, an approach that identifies and quantifies local transcriptomes and proteomes from labelled growth cones of single projections in vivo. Using this approach on the developing callosal projection of the mouse cerebral cortex, we mapped molecular enrichments in tra

SUBMITTER: Poulopoulos A 

PROVIDER: S-EPMC6484835 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature