Photoimmunotherapy targeting prostate-specific membrane antigen: are antibody fragments as effective as antibodies?

ABSTRACT:

Unlabelled

Photoimmunotherapy is a highly cell-selective cancer therapy based on an armed antibody conjugate with a phthalocyanine-based photosensitizer, IR700. Photoimmunotherapy induces rapid and highly specific necrosis in targeted cancer cells after exposure to near-infrared (NIR) light. Cells not expressing the antigen are not affected. To date, photoimmunotherapy has been demonstrated only with full antibody-IR700 conjugates. In this study, small and bivalent antibody fragments, including anti-prostate-specific membrane antigen (PSMA) diabody (Db) and minibody (Mb), were compared with intact IgG for their effectiveness as photoimmunotherapy agents.

Methods

Radioiodinated antibody and antibody fragments with (125)I were used to determine the timing of maximum binding of

SUBMITTER: Watanabe R 

PROVIDER: S-EPMC6484861 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature