Mapping and identification of CsUp, a gene encoding an Auxilin-like protein, as a putative candidate gene for the upward-pedicel mutation (up) in cucumber.

ABSTRACT:

Background

Pedicel orientation can affect the female flower orientation and seed yield in cucumber. A spontaneous mutant possessing upward growth of pedicels was identified in the wild type inbred strain 9930 and named upward-pedicel (up). The morphological and genetic analyses of up were performed in this study. In order to clone the up gene, 933 F₂ individuals and 524 BC₁ individuals derived from C-8-6 (WT) and up were used for map-based cloning.

Results

up was mapped to a 35.2 kb physical interval on chromosome 1, which contains three predicted genes. Sequencing analysis revealed that a 5-bp deletion was found in the second exon of Csa1G535800, and it led to a frameshift mutation resulting in a premature stop codon. The candidate gene of CsUp (Csa1G5

SUBMITTER: Sun J 

PROVIDER: S-EPMC6485165 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature