Polymerase Chain Reaction using "V" Shape Thermal Cycling Program.

ABSTRACT: Polymerase chain reaction (PCR) is the most commonly used technique in molecular biology and diagnostics. To achieve faster PCR reaction time, two strategies were employed by previous studies. That includes improving the thermal ramp rate by developing novel devices to reduce the time wasted on temperature transitions and cutting the holding time in every step, which could even lead to compromise in amplification efficiency. Hence the need to further improve the technique. Methods: A different way to achieve fast DNA amplification is developed by using the previously thought wasted time spent on heating and cooling the samples to finish the amplification. That means the holding time of the three procedures are omitted and this could be carried out on the ordinary PCR thermal cyclers

SUBMITTER: Chen R 

PROVIDER: S-EPMC6485190 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature