Determination of genetic relatedness from low-coverage human genome sequences using pedigree simulations.

ABSTRACT: We develop and evaluate methods for inferring relatedness among individuals from low-coverage DNA sequences of their genomes, with particular emphasis on sequences obtained from fossil remains. We suggest the major factors complicating the determination of relatedness among ancient individuals are sequencing depth, the number of overlapping sites, the sequencing error rate and the presence of contamination from present-day genetic sources. We develop a theoretical model that facilitates the exploration of these factors and their relative effects, via measurement of pairwise genetic distances, without calling genotypes, and determine the power to infer relatedness under various scenarios of varying sequencing depth, present-day contamination and sequencing error. The model is validated by a

SUBMITTER: Martin MD 

PROVIDER: S-EPMC6485253 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature