Enhanced Performance of Next-Generation Sequencing Diagnostics Compared With Standard of Care Microbiological Diagnostics in Patients Suffering From Septic Shock.

ABSTRACT:

Objectives

Culture-based diagnostics represent the standard of care in septic patients, but are highly insensitive and in many cases unspecific. We recently demonstrated the general feasibility of next-generation sequencing-based diagnostics using free circulating nucleic acids (cell-free DNA) in plasma samples of septic patients. Within the presented investigation, higher performance of next-generation sequencing-based diagnostics was validated by comparison to matched blood cultures.

Design

A secondary analysis of a prospective, observational, single-center study.

Setting

Surgical ICU of a university hospital and research laboratory.

Patients

Fifty patients with septic shock, 20 uninfected patients with elective surgery as control cohort.

Interventions

SUBMITTER: Grumaz S 

PROVIDER: S-EPMC6485303 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature