A Novel Microscopic Assay Reveals Heterogeneous Regulation of Local Endothelial Barrier Function.

ABSTRACT: Blood vessels are covered with endothelial cells on their inner surfaces, forming a selective and semipermeable barrier between the blood and the underlying tissue. Many pathological processes, such as inflammation or cancer metastasis, are accompanied by an increased vascular permeability. Progress in live cell imaging techniques has recently revealed that the structure of endothelial cell contacts is constantly reorganized and that endothelial junctions display high heterogeneities at a subcellular level even within one cell. Although it is assumed that this dynamic remodeling is associated with a local change in endothelial barrier function, a direct proof is missing mainly because of a lack of appropriate experimental techniques. Here, we describe a new assay to dynamically measure loc

SUBMITTER: Klusmeier N 

PROVIDER: S-EPMC6486479 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature