Redefining the Practical Utility of Blood Transcriptome Biomarkers in Inflammatory Bowel Diseases.

ABSTRACT:

Background and aims

The study investigates the practical utility of whole-blood gene expression profiling to diagnose inflammatory bowel diseases [IBDs].

Methods

The discovery cohorts included 102 and 51 paediatric IBD patients and controls, and 95 and 46 adult IBD patients and controls, respectively. The replication cohorts included 447 and 76 paediatric IBD patients and controls, and 271 and 108 adult IBD patients and controls, respectively. In the discovery phase, RNA samples extracted from whole peripheral blood were analysed using RNA-Seq, and the predictive values of selected biomarkers were validated using quantitative polymerase chain reaction [qPCR].

Results

In all, 15 differentially expressed transcripts [adjusted p ≤0.05] were selected from the discovery se

SUBMITTER: Ostrowski J 

PROVIDER: S-EPMC6486489 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature