Pumpkin (Cucurbita ficifolia Bouche) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression.

ABSTRACT: Prevention and management of obesity through dietary modification is one of the top way to trim down its consequences. Development of adipose tissue requires the differentiation of less specialized cells, such as human mesenchymal stem cells (hMSCs), into adipocytes. Since food constituents play a major role in the cell differentiation and proliferation, we sought to determine if various extracts of Cucurbita ficifolia (C. ficifolia), could affect the adipogenic differentiation of hMSCs. Flow cytometry analysis with quantitative and qualitative Nile red, and quantitative PCR methods were employed to evaluate the C. ficifolia effect on hMSCs adipogenesis. Results revealed that, chloroform extract exhibits significant adipogenic inhibition than that of hexane and methano

SUBMITTER: Alshammari GM 

PROVIDER: S-EPMC6486525 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature